Effect of epigenotoxic chemicals on DNA methylation profiles of human induced pluripotent stem cells [methylation]. Effect of epigenotoxic chemicals on DNA methylation profiles of human induced pluripotent stem cells [methylation]

We studied the effects of chemical exposure on global DNA methylation by using mCherry-methyl-CpG-binding domain (MBD)-nls human induced pluripotent stem cells (iPSCs). According to the fluorescent MBD parameters, we divided the effects of 135 chemical on global DNA methylation into 3 categories; reduction (hypomethylation), increase (hypermethylation) and little/no effect. We performed DNA methylome assay to examine the effects of 6 epigenotoxic chemicals on genome-wide DNA methylation patterns in human iPSCs. Our results indicated that hypermethylation agents increased DNA methylation and downregulated the expression of genes involved in cell cycle or development. Overall design: Human iPSCs were exposed to DMSO, biotin, etoposide, propyl gallate, theophylline, bisphenol A, and thalidomide for 48 hours and then total genomic DNA was isolated for DNA methylome assay.