Kmt2a and Kmt2b Control Memory Function via Different Transcriptional Programs

We have analyzed changes in histone 3 lysine 4 methylation (H3K4me) and gene expression resulting either from Kmt2a or Kmt2b knockdown in adult hippocampal CA neurons in mice. We implemented Cre/loxP-mediated conditional knockout strategy in order to knockdown either of these histone methyltransferases in excitatory forebrain neurons in adult mice. Examination of H3K4me3 and H3K4me1 levels, and gene expression in hippocampal CA neurons of of adult Kmt2a cKO and Kmt2b cKO mice.本研究针对成年小鼠海马CA区神经元展开分析，探究了Kmt2a或Kmt2b敲低所引发的组蛋白3赖氨酸4甲基化（histone 3 lysine 4 methylation, H3K4me）与基因表达变化。我们采用Cre/loxP介导的条件性敲除（conditional knockout, cKO）策略，以在成年小鼠的兴奋性前脑神经元中敲低这两种组蛋白甲基转移酶中的任意一种。随后我们对成年Kmt2a cKO与Kmt2b cKO小鼠海马CA区神经元中的H3K4me3、H3K4me1水平及基因表达进行了检测。