57Fe Wall Loss Experiment data collected as part of a method development study investigating the precipitation and wall loss of labeled 57Fe when added to M9 Minimal Media

<p>This data was collected as part of a method development study investigating the precipitation and wall loss of labeled ⁵⁷Fe when added to M9 Minimal Media, which is used to grow the model bacterial species <em>Escherichia coli.</em> The bulk media was prepared with the same components and a portion was treated with Chelex-100 to remove metals, while the other portion remained un-chelexed. Half of each treatment was spiked with labeled ⁵⁷Fe and either 0.2 μm or 0.02 μm filtered for comparison of the dissolved and soluble fractions. The ⁵⁷Fe content was monitored over four time points for one week in a shaking incubator, under the same conditions used to culture <em>E. coli</em> for labeling experiments.</p>

本数据集采集自一项方法开发研究，该研究旨在探究标记态⁵⁷铁（⁵⁷Fe）添加至M9基础培养基(M9 Minimal Media)后的沉淀与管壁附着损耗情况；该培养基用于培养模式细菌物种大肠杆菌（Escherichia coli）。批量制备的基础培养基采用统一组分，其中一部分经Chelex-100处理以去除金属离子，另一部分则未进行Chelex-100处理。每组处理（即Chelex处理组与未处理组）均取半数培养基进行⁵⁷Fe加标，随后分别以0.2 μm与0.02 μm孔径滤膜过滤，用于对比溶解态与可溶性组分的差异。在与大肠杆菌标记实验一致的培养条件下，于摇床培养箱中连续培养一周，并在四个时间点对培养基中的⁵⁷Fe含量进行监测。