The 99% confidence intervals of parameters and levels of free radicals.

These intervals were calculated for each parameter separately among the sets of parameters that give χ2 below than a fixed threshold [31]. The sets of parameters were found using a stochastic optimization algorithm (Simulated annealing) that minimized the deviation from measured dynamics of NAD+ reduction in the presence and absence of rotenone, and uncoupled and state 4 respiration rates. Only forward rate constants are shown assuming that the reverse constants change proportionally keeping constant the ratio in accordance with respective ΔEm or dissociation constant. The units for rate constants of monomolecular electron transport are s−1. For the reactions where protons are bound the concentration of protons for pH = 7 is included in the constant (k′ = k·[H+]2). These values have the same units s−1. Dissociation rate constants are also expressed in the same units. Since concentrations are expressed in nmol/mg prot, rate constants for the bindings are expressed in s−1·(nmol/mg prot)−1.