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Forty-Four Novel Protein-Coding Loci Discovered Using a Proteomics Informed by Transcriptomics (PIT) Approach in Rat Male Germ Cells.

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https://www.omicsdi.org/dataset/gpmdb/GPM32320003462
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Data from ProteomeXchange, PXD ID: PXD000872. File: SPC_exc02_compil.msf.mgf. Published as part of Biol Reprod. 2014 Sep 10 . From the Abstract: {{i}} Spermatogenesis is a complex process, dependent upon the successive activation and/or repression of thousands of gene products, and ends with the production of haploid male gametes. RNA sequencing of male germ cells in the rat identified thousands of novel testicular unannotated transcripts, named TUTs. Although such RNAs are usually annotated as long non-coding RNAs, it is possible that some of these TUTs code for protein. To test this possibility, we used a "Proteomics Informed by Transcriptomics" (PIT) strategy combining RNA sequencing data with shotgun proteomics analyses of spermatocytes and spermatids in the rat. {{/i}}
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2014-09-12
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