Comparison of MPA regulated gene expression profiles to those regulated by PROG, DHT, DEX. Comparison of MPA regulated gene expression profiles to those regulated by PROG, DHT, DEX

Medroxyprogesterone acetate (MPA) is a progestin that can bind to and activate progesterone, androgen and glucocorticoid receptors. However, it is not known which receptor mediates MPA action in a cellular context where all three of these receptors are co-expressed and functional. This microarray experiment was performed to compare the transcriptomes induced by MPA and the cognate ligands for these receptors ie progesterone (PROG), 5a-dihydrotestosterone (DHT) and dexamethasone (DEX) in breast cancer cells to determine which was most similar to MPA. Overall design: Transcriptome analysis by microarray for ZR-75-1 breast cancer cells treated for 6 hours with 10 nM MPA, PROG, DHT, DEX or vehicle.