miRNA-seq of lungs tissue of swine supplemented with cholecalciferol and calcidiol

Results indicate that both increasing the dose of cholcalciferol and the use of calcidiol in the diet have a significant effect on lung tissue miRNAs compared to using the standard dose of cholecalciferol. The greatest changes in the transcriptome were induced by supplementation with the increased dose of the combination of cholecalciferol and calcidiol. Overall design: Effect of different doses of cholecalciferol and calcidiol on miRNA profiles of lungs tissue of swine. The vitamin D dosage of the groups was as follows : 1 group (control group) 2000 IU cholecalciferol (grower) and 1500 IU cholecalciferol/ kg feed (finisher)- 12 individuals, 2 group 3000IU cholecalciferol (grower) and 2500 IU cholecalciferol/ kg feed (finisher)- 7 individuals, 3 group 2000 IU cholecalciferol + 1000 IU 25(OH)D3 (grower) and 1500 IU cholecalciferol + 1000 IU 25(OH)D3/kg feed (finisher)-8 individuals, 4 group 2000 IU 25(OH)D3 (grower) and 1500 IU 25(OH)D3/kg feed (finisher)- 12 individuals