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CRISPR/Cas9 EGFP disruption assay targeted deep sequencing. Mus musculus strain:N2a.EGFP

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CRISPR-Cas9基因编辑

基因敲除效率评估

数据链接：

https://www.ncbi.nlm.nih.gov/bioproject/PRJNA352856 数据链接链接失效反馈

官方服务：

资源简介：

Deep sequencing of cleaved EGFP target sites in N2a.EGFP cell line

应用场景：

创建时间：

2016-11-08

CRISPR-Cas9基因编辑

sgRNA优化

The current commonly used single-guide RNA (sgRNA) structure has a shortened duplex compared with the native bacterial clustered regularly interspaced short palindromic repeats RNA (crRNA)–transactiva

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Additional file 1: of Reproducibility of CRISPR-Cas9 methods for generation of conditional mouse alleles: a multi-center evaluation

CRISPR-Cas9基因编辑

小鼠遗传模型分析

Table S1. The guide RNA and singlestranded Oligonucleotide DNA sequences, their concentrations and the length of genomic regions floxed (in bp) reportedÂ in this study. (XLSX 29 kb)

Figshare2024-02-15 更新80

Modeling of the Human Alveolar Rhabdomyosarcoma Pax3-Foxo1 Chromosome Translocation in Mouse Myoblasts Using CRISPR-Cas9 Nuclease

染色体易位建模

CRISPR-Cas9基因编辑

Many recurrent chromosome translocations in cancer result in the generation of fusion genes that are directly implicated in the tumorigenic process. Precise modeling of the effects of cancer fusion ge

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Additional file 10 of CRISPR/Cas9 mediated T7 RNA polymerase gene knock-in in E. coli BW25113 makes T7 expression system work efficiently

CRISPR-Cas9基因编辑

T7噬菌体

Additional file 10.

DataCite Commons2024-02-29 更新90

Target-bound SpCas9 complex with TRAC full RNA guide

CRISPR-Cas9基因编辑

结构生物学

Target-bound SpCas9 complex with TRAC full RNA guide

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