Evaluation of the Effect of Bacillus Subtilis and Pediococcus Acidilactici Mix on Serum Biochemistry, Growth Promotation of Body and Visceral Organs in Lohmann Brown Chicks

ABSTRACT Recently the use of antibiotic growth promoter (AGPs) in poultry feed is banned in China, leading to the increasing demand for AGPs substitutes. Probiotics have been considered as a potential substitute. The purpose of this study was to evaluate the effects of probiotic on serum biochemistry, and growth promotion of body weight and visceral organs and in Lohmann Brown chicks aged 0 to 9 weeks. Five hundred and forty 1-day-old male chicks were randomly divided into three groups, with six replicates and 30 chicks in each replicate. The experiment was carried out over 70 days. Dietary treatments were: (1) basal diet alone (control group); (2) basal diet containing 0.1% probiotics (probiotic group); and (3) basal diet containing 0.01% zinc bacitracin in the feed (antibiotic group). The body weight (BW) of probiotic group was significantly higher than that of the control group during the whole trial period (p<0.05). ADG (0~70 d) in the probiotic group and the antibiotic group was higher than that in the control group (p<0.05). The Oliver weight and serum total protein (TP) in the probiotic group was higher than that in the control group on the 63rd day (p<0.05). The length of the duodenum, the weight of the spleen, duodenum and Jejunum in both the probiotic and antibiotic groups were higher than those in the control group (p<0.05). The ileum weight in the probiotic group was significantly higher than those in the control group (p<0.05). The ileum length in the probiotic group was significantly longer than that in both the antibiotic and control groups (p<0.05). Probiotic mix with Bacillus subtilis and Pediococcus acidilactici play a similar role in promoting growth of body and visceral organs, and it is a promising growth promoter for Lohmann Brown chicks (0 to 9 weeks).

摘要 近期，中国已禁止在家禽饲料中使用抗生素生长促进剂（antibiotic growth promoters, AGPs），由此使得抗生素替代品的市场需求持续增长，益生菌被认为是极具潜力的替代品。本研究旨在评估益生菌对0~9周龄罗曼褐壳蛋鸡雏鸡的血清生化指标、体重生长性能以及内脏器官发育的影响。试验选取540只1日龄雄性雏鸡，随机分为3组，每组设6个重复，每个重复含30只雏鸡，试验周期为70天。日粮处理方案如下：(1) 基础日粮组（对照组）；(2) 添加0.1%益生菌的基础日粮组（益生菌组）；(3) 添加0.01%杆菌肽锌的基础日粮组（抗生素组）。试验结果显示：整个试验周期内，益生菌组雏鸡体重均显著高于对照组（p<0.05）；益生菌组与抗生素组的平均日增重（Average Daily Gain, ADG）在0~70日龄阶段均显著高于对照组（p<0.05）。试验第63天时，益生菌组的肝脏重量与血清总蛋白（total protein, TP）水平均显著高于对照组（p<0.05）。益生菌组与抗生素组的十二指肠长度、脾脏重量、十二指肠重量与空肠重量均显著高于对照组（p<0.05）；益生菌组的回肠重量显著高于对照组（p<0.05）；益生菌组的回肠长度显著长于抗生素组与对照组（p<0.05）。由枯草芽孢杆菌与乳酸片球菌复配而成的益生菌制剂，在促进雏鸡体重与内脏器官生长方面效果与抗生素组相当，是适用于0~9周龄罗曼褐壳蛋鸡雏鸡的极具前景的生长促进剂。