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Reporting off-target effects of recombinant engineering using the pORTMAGE system

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NIAID Data Ecosystem2026-05-01 收录
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Here we inserted point mutations into the cusS gene from Escherichia coli K-12 MG1655 using the pORTMAGE recombineering system. We then performed Illumina sequencing and report off-target variants in all mutant strains.

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2023-09-01
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