Methylation level of PPARGC1A, HLA-DQA1 and ADCY3 in pregnant women with or without GDM

Data generation procedurePyrosequencing Pyrosequencing was used to detect the DNA methylation level of the target gene (PPARGC1A, HLA-DQA1, ADCY3). Bisulfite pyrosequencing was performed on a PyroMark Q96 ID (Qiagen) pyrosequencing system. 40 uL PCR product was mixed, soaked, denaturated and annealed and then reacted with substrate mixture, enzyme mixture and four dNTP in the detector. The weak light device and the processing software in PyroMark Q96 ID (Qiagen) captured and analyzed the specific detection peak formed by the synthetic visible light during the sequencing reaction to achieve the purpose of real-time DNA sequence determination.The blood samples were collected from pregnant women with GDM who were recruited from May and December 2019 in a women’s and children’s hospital located in Southwest China.Tabular dataThe total number of entries was 180. The row headers were:Well: location of sampleAssay: CpG site in geneSample ID: the number of sampleMeth. (%): the methylation level of CpG siteNumber of included CpGs: the total number of CpG site in one geneMean: the mean percentage of methylation level of total CpG site in one gene