Spatial distribution and driving factors of microbial necromass carbon in coastal wetlands of China

Microbial necromass carbon (MNC) constitutes a vital component of soil carbon pools and is integral to the global soil carbon cycle. However, the mechanisms driving MNC accumulation in large-scale coastal wetlands, particularly across different habitat types (such as mangroves, saltmarshes, and mudflats), remain poorly understood. In this study, we conducted extensive sampling across 101 sites in China's coastal wetlands, covering a latitudinal gradient of over 2,500 km, to investigate variations in MNC and its drivers. Additionally, we integrated global data from farmland, forest, grassland, and coastal wetland habitats for a comparative analysis to explore the specificity of carbon storage mechanisms in coastal wetlands. Our results reveal significant differences in MNC content among the three coastal wetland habitats, with mangroves exhibiting the highest MNC levels and saltmarshes the lowest. We found that primary productivity, especially plant biomass, is the dominant driver of MNC..., Soil samples were sampled from 101 sampling sites in Binhai, China. The hydrolysates of the collected soil samples were centrifuged, neutralized, and then centrifuged at 4000 rpm for 10 min. The supernatant was freeze-dried, purified with anhydrous methanol, derivatized with derivatization reagents, and extracted with dichloromethane. The organic phase containing the aminosugar derivative was dried with Nitrogen at 45°C and redissolved in a 1:1 solvent of hexane and ethyl acetate. We quantified the concentrations of GluN, mannosamine (ManN), galactosamine (GalN), and MurA using an Agilent 6890A gas chromatograph/mass spectrometer and a DB-5 capillary column (30 m × 0.25 mm × 0.25 μm) with myo-inositol as an internal standard., , # Spatial distribution and driving factors of microbial necromass carbon in coastal wetlands of China

[https://doi.org/10.5061/dryad.tqjq2bw85](https://doi.org/10.5061/dryad.tqjq2bw85)

## Description of the data and file structure

### Files and variables

#### File: Microbial\_necromass\_carbon\_dataset\_for\_coastal\_wetlands\_in\_china.xlsx

Data table of microbial necromass carbon in coastal wetlands across China

##### Variables

* \"FNC\": Fungal Necromass Carbon
* \"BNC\": Bacterial Necromass Carbon
* \"MNC\": Microbial Necromass Carbon

#### File: Global\_literature\_synthesis\_data\_on\_Microbial\_necromass\_carbon\_and\_Microbial\_necromass\_carbon\_contribution\_to\_Soil\_Organic\_carbon.csv

This dataset compiles results from a global literature synthesis on microbial necromass carbon (MNC) and its contribution to soil organic carbon (SOC).

##### Variables

* \"No\": Number
* “Citation”: Literature Citations
* “Latitude“ & “Longitude”: The longitude and latitude of the Literature...,

微生物残体碳（Microbial necromass carbon, MNC）是土壤碳库的核心组成部分，亦是全球土壤碳循环的关键环节。然而，大型滨海湿地中驱动MNC累积的机制，尤其是不同生境类型（如红树林、盐沼与潮滩）下的调控路径，仍有待系统阐明。本研究在中国滨海湿地的101个样点开展大范围采样，覆盖超过2500公里的纬度梯度，以探究MNC的空间变异特征及其调控因子。此外，本研究整合了全球农田、森林、草原及滨海湿地生境的相关数据开展对比分析，旨在揭示滨海湿地碳存储机制的特异性。研究结果表明，三类滨海湿地生境的MNC含量存在显著差异：红树林生境的MNC水平最高，盐沼生境最低。本研究发现，初级生产力尤其是植物生物量是调控MNC的主导因子……土壤样品采自中国滨海地区的101个采样点。对采集的土壤样品水解液依次进行离心、中和处理后，以4000 rpm转速离心10分钟。将上清液冷冻干燥，经无水甲醇纯化、衍生化试剂衍生后，用二氯甲烷萃取。将含有氨基糖衍生物的有机相在45℃下以氮气吹干，随后用正己烷与乙酸乙酯体积比1:1的混合溶剂复溶。本研究采用安捷伦（Agilent）6890A气相色谱-质谱联用仪，搭配DB-5毛细管柱（30 m × 0.25 mm × 0.25 μm），以肌醇为内标，定量测定了氨基葡萄糖（GluN）、甘露糖胺（ManN）、半乳糖胺（GalN）及胞壁酸（MurA）的浓度。

# 中国滨海湿地微生物残体碳的空间分布及其调控因子
https://doi.org/10.5061/dryad.tqjq2bw85

## 数据与文件结构说明
### 文件与变量
#### 文件：Microbial_necromass_carbon_dataset_for_coastal_wetlands_in_china.xlsx
中国滨海湿地微生物残体碳数据集表格
##### 变量
* "FNC"：真菌残体碳（Fungal Necromass Carbon）
* "BNC"：细菌残体碳（Bacterial Necromass Carbon）
* "MNC"：微生物残体碳（Microbial necromass carbon）

#### 文件：Global_literature_synthesis_data_on_Microbial_necromass_carbon_and_Microbial_necromass_carbon_contribution_to_Soil_Organic_carbon.csv
本数据集整合了全球范围内关于微生物残体碳（MNC）及其对土壤有机碳（Soil Organic Carbon, SOC）贡献的文献综合研究结果。
##### 变量
* "No"：编号
* "Citation"：文献引用信息
* "Latitude"与"Longitude"：文献对应样点的经纬度……