CIL:40235, Mus musculus, CNS neuron (sensu Vertebrata), glial cell, blood vessel endothelial cell. In Cell Image Library

The animal was perfused with Ringer's solution for 2 minutes followed by DiI for 10 minutes, and then 2% paraformaldehyde for 10 minutes. The brain was post-fixed overnight, and the tissue subsequently sectioned. After buffer washes, sections were stained with Fluoromyelin (1:100 overnight in cold room), rinsed with buffer washes, and subsequently stained TO-PRO3 (1:3000) for 45 minutes at room temp, rinsed and mounted using Gelvatol. 16 z-planes at 5 µm intervals across the entire section used to generate the tiled 3-D mosaic, acquired with an Olympus Fluoview 1000.