The BCG Delta BCG1419c vaccine candidate reduces lung pathology, IL-6, TNFa, and IL-10 during chronic TB infection

Comparison of transcriptional differences (RNA-seq) between BCG delta1419c and wild-type BCG that might explain the pathological differences in vaccination studies. Cultures of wild-type (Wt) and Delta1419c were grown for 10 days and harvested for mRNA expression profiling by Illumina sequencing, in duplicate from two independent experiments. The sequence reads that passed quality filters were aligned to BCG reference genome using STAR, followed by read count collection by HTSeq and differential expression analysis with DESeq2.