Use of pro-biotics in Striped Trumpeter culture

This data represents research conducted as part of a PhD project on Striped Trumpeter (Latris lineata).
Knowledge of the microbial bacterial community of the larvae, seawater and live feeds is necessary in order to develop an understanding of what represents a healthy microbial ecology relevant for larval fish rearing success. 16S ribosomal RNA (rRNA) gene-based clone library and terminal restriction fragment length polymorphism (TRFLP) analyses were utilized to examine the microbial community associated with larvae cultured under different “greenwater” conditions and determine the success of probiont application in live feeds used for larval fish rearing. Though water quality was apparently influential on larval survival, no specific bacterial species present either on the larvae or in surrounding tank water could be attributed to reduced survival. Two bacterial strains, Pseudoalteromonas sp. ST8 and Vibrio sp. ST7 were found to inhibit bacteria pathogenic to larval fish through production of extracellular substances. Strain ST8 was found to be compatible with artemia, rotifers and larval fish and outcompeted the fish pathogen Vibrio proteolyticus in competition experiments. Encapsulation experiments indicated strain ST8 was transferrable to artemia and thus represent a potential probiont for larval fish mariculture.

本数据集源自一项针对条纹突吻鲈（Striped Trumpeter, Latris lineata）的博士研究项目。明确仔鱼、海水及鲜活饵料的微生物细菌群落组成，是理解与仔鱼繁育成功相关的健康微生物生态的必要前提。本研究采用基于16S核糖体RNA（16S ribosomal RNA, rRNA）基因的克隆文库及末端限制性片段长度多态性（TRFLP）分析技术，对不同“绿水（greenwater）”养殖条件下培育的仔鱼相关微生物群落进行考察，并评估用于仔鱼繁育的鲜活饵料中益生菌施用效果。尽管水质对仔鱼存活率存在显著影响，但并未发现仔鱼体表或养殖槽水体中的特定细菌类群与存活率下降存在关联。研究发现两株细菌——假交替单胞菌属（Pseudoalteromonas）菌株ST8与弧菌属（Vibrio）菌株ST7——可通过分泌胞外物质抑制对仔鱼具有致病性的细菌。实验表明菌株ST8可与卤虫（artemia）、轮虫（rotifers）及仔鱼兼容，且在竞争实验中相较于鱼类致病菌溶蛋白弧菌（Vibrio proteolyticus）更具竞争优势。包囊实验结果显示，菌株ST8可转移至卤虫体内，因此具备成为仔鱼海水养殖潜在益生菌的应用潜力。