Data from: Large effect quantitative trait loci for salicinoid phenolic glycosides in Populus: Implications for gene discovery

Genomic studies have been used to identify genes underlying many important plant secondary metabolic pathways. However, genes for salicinoid phenolic glycosides (SPGs)—ecologically important compounds with significant commercial, cultural, and medicinal applications—remain largely undescribed. We used a linkage map derived from a full‐sib population of hybrid cottonwoods (Populus spp.) to search for quantitative trait loci (QTL) for the SPGs salicortin and HCH‐salicortin. SSR markers and primer sequences were used to anchor the map to the V3.0 P. trichocarpa genome. We discovered 21 QTL for the two traits, including a major QTL for HCH‐salicortin (R2 = .52) that colocated with a QTL for salicortin on chr12. Using the V3.0 Populus genome sequence, we identified 2,983 annotated genes and 1,480 genes of unknown function within our QTL intervals. We note ten candidate genes of interest, including a BAHD‐type acyltransferase that has been potentially linked to PopulusSPGs. Our results complement other recent studies in Populus with implications for gene discovery and the evolution of defensive chemistry in a model genus. To our knowledge, this is the first study to use a full‐sib mapping population to identify QTL intervals and gene lists associated with SPGs.

基因组学研究已被用于鉴定众多重要植物次生代谢通路的关键基因。然而，水杨苷类酚苷（salicinoid phenolic glycosides, SPGs）作为兼具重要商业、文化与药用价值的生态活性化合物，其相关合成基因至今仍未得到充分解析。本研究利用源自杂交杨（杨属*Populus* spp.）全同胞群体的连锁图谱，对SPGs中的水杨苷（salicortin）与HCH-水杨苷（HCH-salicortin）的数量性状位点（quantitative trait loci, QTL）进行定位分析。本研究使用简单序列重复（simple sequence repeat, SSR）标记与引物序列，将该连锁图谱锚定至毛果杨*P. trichocarpa* V3.0版本参考基因组。本研究共鉴定出调控这两个性状的21个QTL，其中HCH-水杨苷的主效QTL（决定系数R²=0.52）与12号染色体上调控水杨苷的QTL存在共定位。依托毛果杨V3.0基因组序列，本研究在上述QTL区间内共鉴定出2983个已注释基因与1480个功能未知基因。本研究还筛选出10个候选功能基因，其中包括一个此前被推测与杨属SPGs合成相关的BAHD型酰基转移酶。本研究结果可为杨属这一模式属的基因发掘与防御性次生代谢演化研究提供补充，同时也为近期相关杨属研究提供了新的佐证。据我们所知，本研究是首次利用全同胞作图群体定位与SPGs相关的QTL区间并筛选出关联基因列表的研究。