Quantitative live imaging reveals PRICKLE1 controls junctional neural tube morphogenesis independent of Planar Cell Polarity

This dataset contains the raw images and code generated for a study of how PRICKLE1 (PK1) controls morphogenesis of the junctional neural tube in a transgenic quail model.
The data includes imaging of Lifeact-EGFP, wildtype and nuc-mCherry-membrane-EGFP quail embryos with and without perturbations including PK1 knockdown, FGF inhibition, SLUG knockdown and appropriate controls, and immunofluorescent labelling of extracellular matrix components, Myosin Light Chain and members of the Planar Cell Polarity pathway.
Code is provided for projection of 3D data to 2D, quantification of cell area and apical PK1 intensity and generation of weighted polar histograms and rose plots.