MS2-affinity purification coupled with RNA sequencing (MAPS) reveals GcvB sRNA targetome.

Recently, we developed an in vivo technology to draw the interacting map of a specific small regulatory RNA (sRNA). We called it MAPS for MS2-affinity purification coupled with RNA sequencing. Using this technology, we already revealed the targetome of RyhB, RybB and DsrA, three well-characterized sRNAs in Escherichia coli. In this study, we perform MAPS with GcvB, a sRNA involved in amino acid metabolism. Overall design: Identification of RNAs co-purified with MS2-GcvB in a rne131 ?gcvB strain. GcvB (without MS2) was used as control.