Kinetic parameters used for the simulations in fig. 3.

The interconversion rates correspond to the fully-liganded state. The free energy gained by the binding of a ligand is assumed to be used to speed-up forward rates and slow down reverse rates. Therefore the forward rate with n ligands bound are obtained by multiplying the rate with n+1 ligands bound by the respective c constant (see Table 1) to the power of the transition parameter. The reverse rates are obtained by dividing with the c constant to the power of the transition parameter. We assumed an even distribution of free energy and used a transition parameter of 0.5 for all transitions. See [31] for an example of non even distribution.