Gene expression in Arabidopsis leaves after P. brassicae egg deposition

Characterization of Arabidopsis thaliana`s transcriptional response to Pieris brassicae eggs Arabidopsis thaliana Col-0 wild type plants were grown under short day conditions at 20°C for seven weeks (6 weeks: 10 h/14 h light/dark + 1 week: 8 h/ 16 h light/dark). Seven week old plants were (i) deposited with eggs (one egg cluster with about 40 eggs was deposited onto the lower surface of a rosette leaf within position 14-17) or were (ii) left untreated. Six days after egg deposition, leaf tissue was harvested from egg-deposited and untreated plants. The tissue was harvested from a leaf region proximal to the egg deposition site.