I2K2022 GliaMorph: A Tool to Quantify Glia Morphology in 3D

Dataset for the I2K2022 Workshop "<strong>GliaMorph: A Tool to Quantify Glia Morphology in 3D</strong>" presented by Elisabeth Kugler, Sara Beqiri, Karim Nizam, Eva-Maria Breitenbach, and Ryan B MacDonald. <strong>Code</strong>. The code used for the analysis is the GliaMorph toolkit (https://github.com/ElisabethKugler/GliaMorph). <strong>Samples</strong>. Experiments performed at UCL conformed to UK Home Office regulations and were performed under Home Office Project Licence PP2133797 held by RM. Maintenance of adult <em>Tg(TP1bglob:VenusPest)^s940</em> zebrafish in the fish facilities was conducted according to previously described husbandry standard protocols at 28°C with a 14:10 hours (h) light:dark cycle. Embryos, obtained from controlled pair- or group-mating, were incubated in E3 buffer with methylene blue and 0.1% PTU applied between 10-24hpf and refreshed at a minimum of every 24h. <strong>Acquisition</strong>. Zeiss LSM 900 with a 40x water-immersion LD C-Apochromat (NA 1.1). Laser lines 488 nm. <strong>Data</strong>. Three example tiffs of Muller Glia in the retina of 3 day old zebrafish. Folder contains data analysed with the GliaMorph toolkit. Data used for training/teaching purposes.本数据集为伊丽莎白·库格勒（Elisabeth Kugler）、萨拉·贝基里（Sara Beqiri）、卡里姆·尼扎姆（Karim Nizam）、伊娃-玛丽亚·布赖滕巴赫（Eva-Maria Breitenbach）与瑞安·B·麦克唐纳（Ryan B MacDonald）在I2K2022工作坊上展示的**GliaMorph：一款用于三维胶质细胞形态量化的工具**。**代码**：本次分析所用代码为GliaMorph工具包（https://github.com/ElisabethKugler/GliaMorph）。**样本**：于伦敦大学学院（University College London, UCL）开展的实验符合英国内政部（UK Home Office）相关规定，实验依托RM持有的内政部项目许可PP2133797完成。成年<em>Tg(TP1bglob:VenusPest)^s940</em>斑马鱼的饲养严格遵循此前报道的标准饲养流程，饲养温度设置为28℃，光暗周期为14小时光照:10小时黑暗。通过可控配对或分组交配获得的胚胎置于添加亚甲基蓝的E3缓冲液中培养，并在受精后10-24小时（hours post fertilization, hpf）期间添加0.1%的PTU，且至少每24小时更换一次培养液。**成像采集**：使用蔡司（Zeiss）LSM 900共聚焦显微镜，搭配40倍水浸式LD C-Apochromat物镜（数值孔径NA 1.1），激发采用488 nm激光线。**数据**：包含3例3日龄斑马鱼视网膜穆勒胶质细胞（Muller Glia）的TIFF格式文件。该文件夹内包含经GliaMorph工具包分析后的数据，本数据集仅用于训练与教学用途。