Optimization data for determination of varenicline through its reaction with erythrosine

Varenicline is used along with education and counseling to help people stop smoking. Erythrosine B, the food additive has been recently investigated as a fluorescent dye for the determination of drugs. Herein, two new facile methods were examined for varenicline, the smoking cessation aid using this fluorochrome. In the first method; the fluorescence of erythrosine B was quenched by increasing concentrations of varenicline through ion-pair complex formation. This linear response was a basis for the spectrofluorimetric method for varenicline quantitation in pure and dosage forms. The quenching is correlated with the concentration linearly over the range of 0.4-4.0 μg/mL at 550 nm after excitation at 528 nm with a correlation coefficient of 0.9993. Different parameters were investigated to reach the optimal conditions with highest sensitivity and repeatability. The second method is depending on measuring the formed complex spectrophotometrically at 550 nm over the range of 1.0-10.0 μg/mL ...伐尼克兰（Varenicline）常与健康教育及咨询干预联用，用于辅助人群戒烟。食品添加剂赤藓红B（Erythrosine B）近年来被研究作为荧光染料，应用于药物含量测定。本文针对戒烟辅助药伐尼克兰，基于该荧光染料开发并考察了两种简便分析方法。第一种方法基于离子对复合物形成原理：随着伐尼克兰浓度升高，赤藓红B的荧光发生猝灭。该荧光强度与药物浓度呈线性响应，以此建立了纯品及制剂中伐尼克兰的荧光分光光度定量方法。在528 nm激发波长、550 nm检测波长下，该方法在0.4~4.0 μg/mL浓度范围内线性良好，相关系数达0.9993。本研究考察了多项实验参数，以优化得到灵敏度与重现性最优的实验条件。第二种方法则通过分光光度法测定所形成的复合物吸光度，检测波长为550 nm，线性范围为1.0~10.0 μg/mL……