Four-species co-culture of Novosphingobium sp. G5, Pseudomonas sp. G7, Flavobacterium sp. G16 and Microbacterium sp. D12 Metagenomic assembly

In order to dissect the molecular mechanism of bacteria synergy in co-culture. We compared the meta-transcriptome of four-species co-culture with single species monocultures.Exponential cultures of Sphingomonas sp. G5, Pseudomonassp. G7, Flavobacterium sp. G16 and Microbacterium sp. D12were adjusted to an optical density at 600 nm of 0.15 in tryptic soy broth(TSB) medium. One milliliter of each culture and a mixture of them, whichcontains 250 µL of each, were then inoculated into 50 mL fresh TSB medium.After 12 h incubation at 25 ? with shaking (200 rpm), cells were collected bycentrifugation at 4000× g. Total RNA was extracted with TRIzol reagent(Invitrogen) following the manufacturer’s instructions. RNA-seq librariesconstructed with co-culture RNA and the control RNA, which is a mixture of onemicrogram of each single species RNA, were sequenced by HiSeq 2500 platform with standard protocols (BGI, China).