Hepatitis B virus sero-profiles and genotypes in HIV-1 infected and uninfected injection and non-injection drug users from coastal Kenya

Background: Information about HBV sero-markers, infection stages and genotypes in HIV-1 infected and uninfected injection and non-injection drug users (IDUs) in Kenya remains elusive. Methods: A cross-sectional study examining HBV sero-marker, infection stages and genotypes was conducted among HIV-1 infected and uninfected, respectively, IDUs (n = 157 and n = 214) and non-IDUs (n = 139 and n = 48), and HIV-1 uninfected non-drug using controls (n = 194) from coastal, Kenya. HBV sero-marker and infection stages were based on HBV 5-panel rapid test plasma sero-reactivity. DNA was extracted from acute and chronic plasma samples and genotypes established by nested-PCR and direct sequencing. Results: HBsAg positivity was higher in HIV-1 infected IDUs (9.6 %) relative to HIV-1 uninfected IDUs (2.3 %), HIV-1 infected non-IDUs (3.6 %), HIV-1 uninfected non-IDUs (0.0 %) and non-drug users (2.6 %; P = 0.002). Contrastingly, HBsAb positivity was higher in HIV-1 uninfected IDUs (14.6 %) and non-IDUs...

背景：目前关于肯尼亚人类免疫缺陷病毒1型（HIV-1）感染者与未感染者、注射吸毒者（IDUs）及非注射吸毒者的乙型肝炎病毒（HBV）血清标志物、感染分期及基因型的相关信息仍较为匮乏。方法：本研究为横断面研究，招募了来自肯尼亚沿海地区的研究对象，按分组分别为：人类免疫缺陷病毒1型（HIV-1）感染者与未感染者注射吸毒者（IDUs）（例数分别为157例与214例）、非注射吸毒者（例数分别为139例与48例），以及未感染HIV-1的非吸毒对照人群（194例）。乙型肝炎病毒（HBV）血清标志物及感染分期通过HBV五联快速检测试剂盒的血浆血清反应性结果判定。从急性与慢性血浆样本中提取DNA，并通过巢式聚合酶链反应（nested-PCR）与直接测序技术确定基因型。结果：人类免疫缺陷病毒1型（HIV-1）感染者注射吸毒者（IDUs）的乙型肝炎病毒表面抗原（HBsAg）阳性率（9.6%）高于HIV-1未感染者注射吸毒者（2.3%）、HIV-1感染者非注射吸毒者（3.6%）、HIV-1未感染者非注射吸毒者（0.0%）及非吸毒人群（2.6%；P=0.002）。与之相反，HIV-1未感染者注射吸毒者（14.6%）与非注射吸毒者的乙型肝炎病毒表面抗体（HBsAb）阳性率更高……