Multi-colour super-resolution images of untreated and rifampicin-treated Xenorhabdus doucetiae (LB, exponential phase)

This dataset and CARE model is part of the publication "Transertion and cell geometry organize the Escherichia coli nucleoid during rapid growth". It contains all SMLM images that were used for the publication, as well as the single-cell regions of interest for analyses. Xenorhabdus doucetiae chromosomally expressing MreBsw-sfGFP were grown to exponential phase in LB Lennox and antibiotics were added for 0-30 min. Cultures were then chemically fixed, permeabilised and imaged for the nucleoid (JF646-Hoechst) and membranes (Nile Red) using PAINT. More information can be found in the publication.本数据集与CARE模型均来自发表论文"Transertion and cell geometry organize the Escherichia coli nucleoid during rapid growth"。该数据集包含该论文所用的全部单分子定位显微镜（Single-Molecule Localization Microscopy, SMLM）成像数据，以及用于分析的单细胞感兴趣区域。实验中，将染色体表达MreBsw-sfGFP的杜氏发光杆菌（Xenorhabdus doucetiae）接种于LB Lennox培养基中培养至指数生长期，随后添加抗生素处理0~30分钟。随后对培养物进行化学固定与透化处理，并采用纳米尺度形貌点积累成像（Points Accumulation for Imaging in Nanoscale Topography, PAINT）技术，以JF646-Hoechst标记类核、尼罗红标记细胞膜完成成像。更多详细信息可参阅该论文。