The Process Of Hdac11 Assay Development: Effect Of Dmso

For the purpose of compound library screenings, the compounds would be made available in DMSO and thus, when they would be incubated with the protein, the protein solution will now contain DMSO as well. It is important to determine the concentration of DMSO that could be tolerated by the protein such that the protein stays active. Hence, the effect of varying concentration of DMSO was analyzed on HDAC11 activity.为开展化合物库筛选实验，所有化合物均以二甲基亚砜（DMSO）为溶剂配制，因此当此类化合物与蛋白质共孵育时，蛋白质溶液中也将含有DMSO。明确可维持蛋白质活性的DMSO耐受浓度至关重要，为此本研究分析了不同浓度DMSO对组蛋白去乙酰化酶11（HDAC11）活性的影响。