Metabolic effects of cesarean section induced gut microbial dysbiosis in high-fat diet induced obese mice

The aim of this study was to investigate the causative effect of CS induced dysbiosis on obesity and insulin resistance in a high-fat diet induced obese (DIO) mouse model. Male germ-free BALB/c mice were humanized by fecal microbiota transfer using samples from children born by CS or VD and fed HFD for 16 weeks. Adipose tissue was sampled for RNAseq at study termination. Overall design: Male mice were either inoculated with GM from vaginally born infants (HM_VD) or cesarian section born infants (HM_CS) anf then subjectesd to 16 week HFD feeding