落叶松EST-SSR标记的开发及其多态性和跨种扩增评价
收藏国家林业和草原科学数据中心2022-11-02 更新2024-03-06 收录
下载链接：
https://www.forestdata.cn/dataDetail.html?id=CSTR:17575.11.0220221102151.040001.V1
下载链接
链接失效反馈官方服务：
资源简介：
Prince Rupprecht’s larch (Larix principis-rupprechtii Mayr) is an important deciduous conifer species that has been widely planted in North China due to its major ecological and commercial value. However, the paucity of genomic data and robust molecular markers has hampered genetic and genomic studies. Here, transcriptome sequencing of L. principis-rupprechtii callus was performed using the Illumina platform. By mining 43,753 assembled unigenes, 1418 expressed sequence tagsimple sequence repeats (EST-SSRs) derived from 1300 unigenes were identified. A total of 1065 primer pairs were designed and 240 of these selected at random for validation among 24 L. principis-rupprechtii individuals. Of these, 52 primer pairs were scored as polymorphic, and 20 polymorphic EST-SSR markers were further selected to genotype 66 clones deployed in a clonal seed orchard of L. principis-rupprechtii.
提供机构：
国家林业和草原科学数据中心
创建时间：
2022-11-02
