Profiling of CD14+ monocytes from paired rheumatoid arthritis (RA)-patient peripheral blood and synovial fluid samples

CD14+ monocytes sorted from the synovial fluid or peripheral blood of rheumatoid arthritis patients were analyzed by full transcriptome microarray analysis. Monocytes from healthy control samples (peripheral blood) were also profiled. A total of 26 samples consisting of 16 paired and 2 un-paired synovial fluid and peripheral blood derived CD14+ monocytes from RA-patients, as well as peripheral blood CD14+ monocytes from 8 healthy control subjects were compared. Sorted monocytes (at least of 95% purity) from each of the donors were obtained individually, and compared by full transcriptome profiling.