Data for: Reproductive isolating mechanisms contributing to asymmetric hybridization in Killifishes (Fundulus spp.)

When species hybridize, one F1 hybrid cross type often predominates. Such asymmetry can arise from differences in a variety of reproductive barriers, but the relative roles and concordance of pre-mating, post-mating prezygotic barriers, and post-zygotic barriers in producing these biases in natural animal populations have not been widely investigated. Here, we study a population of predominantly F1 hybrids between two killifish species (Fundulus heteroclitus and Fundulus diaphanus) in which >95% of F1 hybrids have F. diaphanus mothers and F. heteroclitus fathers (D♀×H♂). To determine why F. heteroclitus x F. diaphanus F1 hybrids (H♀×D♂) are so rare, we tested for asymmetry in pre-mating reproductive barriers (female preference, male aggression) at a common salinity (10 ppt) and post-mating, pre-zygotic (fertilization success) and post-zygotic (embryonic development time and hatching success) reproductive barriers at a range of ecologically relevant salinities (0, 5, 10, and 15 ppt). ..., , All analyses conduced in R.当物种发生杂交时，通常会有某一种F1杂交（F1 hybrid）组合占据优势。这种杂交不对称性可能源于多种生殖隔离的差异，但目前学界尚未就交配前生殖屏障（pre-mating reproductive barriers）、交配后合子前生殖屏障（post-mating prezygotic barriers）以及合子后生殖屏障（post-zygotic barriers）在自然动物种群中产生此类偏向性时的相对作用与协同效应展开广泛研究。本研究聚焦于两个鳉鱼物种（Fundulus heteroclitus与Fundulus diaphanus）之间以F1杂交后代为主的种群，其中超过95%的F1杂交个体均以F. diaphanus为母本、F. heteroclitus为父本（即D♀×H♂组合）。为探明为何F. heteroclitus与F. diaphanus的F1杂交组合（H♀×D♂）极为罕见，我们分别在恒定盐度（10 ppt）下检测了交配前生殖屏障（雌鱼择偶偏好、雄鱼攻击行为）的不对称性，并在一系列生态相关盐度（0、5、10及15 ppt）下检测了交配后合子前（受精成功率）与合子后（胚胎发育时长及孵化成功率）生殖屏障的不对称性。……所有分析均通过R语言完成。