Illuminating the active virosphere with BONCAT and single virus genomic technologies, Pacific Ocean samples
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Raw reads (forward and reverse) from sorted and MDA-amplified samples from Pacific Ocean are supplied here. The sample labels refer to the type of sample (cellular or viral) and whether it belongs to the BONCAT active fraction or non-active (inactive). For all samples, click reactions (BONCAT reaction) lasts 6 hours. In addition, code indicates whether it came from the Pacific Ocean (PO). CA: active cells; 2 sequencing productsCN: inactive cells; 2 sequencing productsVA: activate viruses; 3 sequencing productsVA2: activate viruses; 2 sequencing productsVN: inactivate viruses; 3 sequencing productsPO: Pacific Ocean samples
本数据集提供了源自太平洋、经分选及多重置换扩增(MDA)处理的样本的正向与反向原始测序读段(Raw reads)。样本标签用于标识样本类型(细胞样本或病毒样本),以及是否属于BONCAT活性组分或非活性(失活)组分。所有样本的点击化学反应(BONCAT反应)均持续6小时。此外,通过编码可标识样本是否来自太平洋(PO)。其中:CA为活性细胞样本,对应2个测序产物;CN为失活细胞样本,对应2个测序产物;VA为活性病毒样本,对应3个测序产物;VA2为活性病毒样本,对应2个测序产物;VN为失活病毒样本,对应3个测序产物;PO代表太平洋来源样本。



