Liquid Chromatography-Mass Spectrometry Method Validation for β-Hydroxythiofentanyl

This dataset corresponds to the method validation process of a Liquid Chromatography-Mass Spectrometry (LC-MS) analytical approach developed for the detection and quantification of β-Hydroxythiofentanyl—a synthetic opioid with high pharmacological activity and potential forensic/toxicological relevance. The dataset encompasses key validation parameters, including linearity (calibration curve data across relevant concentration ranges), accuracy (recovery rates from matrix-matched samples such as plasma, urine, or tissue homogenates), precision (intra-day and inter-day repeatability with relative standard deviations), selectivity (peak purity data and interference testing against structurally similar opioids), limit of detection (LOD) and limit of quantification (LOQ), matrix effect (ion suppression/enhancement assessment), and stability (data on short-term, long-term, freeze-thaw, and post-preparation stability of β-Hydroxythiofentanyl in matrices). Additionally, the dataset includes critical experimental details: LC conditions (column type, mobile phase composition, flow rate, injection volume), MS parameters (ionization mode, collision energy), sample preparation protocols (extraction method, cleanup steps), and replicate measurement data for quality control (QC) samples. This dataset serves as a foundational resource for researchers in forensic toxicology, clinical chemistry, or analytical chemistry seeking to adopt, verify, or optimize LC-MS methods for β-Hydroxythiofentanyl analysis, and supports reproducibility in studies involving this synthetic opioid.

本数据集对应为检测与定量β-羟基硫代芬太尼（β-Hydroxythiofentanyl）所开发的液相色谱-质谱联用法（Liquid Chromatography-Mass Spectrometry, LC-MS）分析方法的方法学验证流程。β-羟基硫代芬太尼是一种兼具高药理活性与法医毒理学研究价值的合成阿片类物质。该数据集涵盖核心方法学验证参数，包括线性（覆盖相关浓度区间的校准曲线数据）、准确度（血浆、尿液或组织匀浆等基质匹配样品的回收率）、精密度（日内与日间重复性及相对标准偏差）、选择性（峰纯度数据及针对结构相似阿片类药物的干扰试验）、检出限（limit of detection, LOD）与定量限（limit of quantification, LOQ）、基质效应（离子抑制/增强效应评估）以及稳定性（基质中β-羟基硫代芬太尼的短期、长期、冻融循环及制备后稳定性数据）。此外，本数据集还收录了关键实验细节：液相色谱条件（色谱柱类型、流动相组成、流速、进样体积）、质谱参数（电离模式、碰撞能量）、样品前处理方案（提取方法、净化步骤），以及质控（QC）样品的平行测定数据。本数据集可为法医毒理学、临床化学或分析化学领域中，旨在采用、验证或优化β-羟基硫代芬太尼分析LC-MS方法的研究人员提供基础支撑资源，并为涉及该合成阿片类物质的相关研究提供可重复性保障。