Pre-fertilisation gamete thermal environment influences reproductive success, unmasking opposing sex-specific responses in Atlantic Salmon

# Data set associated to the research article 'Pre-fertilisation gamete thermal environment influences offspring fitness, unmasking opposing sex-specific responses in Atlantic Salmon #All the material has been deposited on the DRYAD repository for replicability, transparency and for future research purposes. #List of necessary files: Thermal\_switch\_R\_NEWtime01.xlsx Thermal\_switch\_R\_single\_MAY.xlsx Thermalfrypertotmean\_1.xlsx Thermalsingle.xlsx Thermalsingle1.xlsx Thermalsinglefin.xlsx #files containing the collected parameters and variables used in the models. Data are repeated among Thermal\_switch\_R\_NEWtime01.xlsx, Thermal\_switch\_R\_single\_MAY.xlsx, Thermalsingle.xlsx Thermalsingle1.xlsx and Thermalsinglefin.xlsx, however they are re-arranged differently for specific graphical or output purposes. The file Thermalfrypertotmean\_1.xlsx is an output file where specific variables have been summarised for graph input and base stats. R\_script\_Gamete\_responses\_Grazianoetal.txt #(R-script to run all the analyses by using the above mentioned datasets R\_script\_Gamete\_responses\_Grazianoetal\_revised.txt #(R-script to run all the analyses by using the above mentioned datasets (after revisions\_graphs updated). Glossary of variables: TRAY: Tray number in which each sub replicated groups belonging to a specific Family were raised. TREATMENT: Temperature treatment of 2 C or 8 C to which either eggs, sperm or embryos were exposed (e.g. WWW means that both egg prefertilisation temperature, sperm prefertilisation temperature and embryo developmental temperature were set to 8 C. TOTFRY: Total amount of fry hatched FRYD: number of fry per day EGGCOUNT: number of starting egg number MALE: unique #ID for male FEMALE: unique #ID for female TPFtimepoint: reference timepoints every 12 hrs FAMILY: Unique Family #ID EGG: temperature treatment for eggs SPERM: temperature treatment for sperm DEV: temperature treatment for embryonic development FRYPOP: Proportion of fry hatched per day FRYPERTOT: Proportion of fry hatched in total TPF: Time post-fertilisation in days Csum: cumulative sum DEVELOPMENTALT: developmental temperature (warm or cold) MALFNOEYE: number of malformed embryos DEADONHATCH: number of embryos dead on hatch EYEDDEAD: number of embryos dead after reaching the eyed stage MALFNOEYEPROP: proportion of malformed embryos EYEDDEADPROP:proportion of embryo dead after reaching the eyed stage DEADONHATCHPROP: proportion of embryo dead after reaching the eyed stage FRYTOTPROP: proportion of eggs that successfully hatched DDAY: time port-fertilisation expressed in degree days

# 本数据集关联于研究论文《受精前配子热环境影响后代适合度，揭示大西洋鲑（Atlantic Salmon）的性别特异性对立响应》
# 所有研究材料已上传至DRYAD数据仓储，以保障可复制性、透明度，并可供后续研究使用。
# 所需文件清单：
Thermal_switch_R_NEWtime01.xlsx
Thermal_switch_R_single_MAY.xlsx
Thermalfrypertotmean_1.xlsx
Thermalsingle.xlsx
Thermalsingle1.xlsx
Thermalsinglefin.xlsx
# 上述文件包含建模所用的采集参数与变量。其中Thermal_switch_R_NEWtime01.xlsx、Thermal_switch_R_single_MAY.xlsx、Thermalsingle.xlsx、Thermalsingle1.xlsx及Thermalsinglefin.xlsx的数据内容存在重复，但为适配特定绘图或输出需求，数据的排布格式各有不同。文件Thermalfrypertotmean_1.xlsx为输出文件，已针对绘图输入与基础统计需求对特定变量进行了汇总整理。
R_script_Gamete_responses_Grazianoetal.txt # 用于基于上述数据集运行全部分析的R脚本
R_script_Gamete_responses_Grazianoetal_revised.txt # 用于基于修订后的数据集（含更新后的绘图）运行全部分析的R脚本
# 变量释义：
TRAY：承载隶属于特定家系的重复分组的培养托盘编号
TREATMENT：针对卵子、精子或胚胎设置的温度处理条件，分为2℃与8℃两组。例如，WWW表示卵子受精前温度、精子受精前温度与胚胎发育温度均设置为8℃。
TOTFRY：孵化出的幼鲑总数量
FRYD：每日孵化的幼鲑数量
EGGCOUNT：初始卵子总数
MALE：雄性个体唯一编号标识
FEMALE：雌性个体唯一编号标识
TPFtimepoint：每12小时记录一次的参考时间节点
FAMILY：家系唯一编号标识
EGG：卵子的温度处理条件
SPERM：精子的温度处理条件
DEV：胚胎发育阶段的温度处理条件
FRYPOP：每日孵化幼鲑占总孵化量的比例
FRYPERTOT：总孵化幼鲑占初始卵子数的比例
TPF：受精后天数
Csum：累计求和值
DEVELOPMENTALT：发育阶段温度（温性或寒性）
MALFNOEYE：畸形胚胎数量
DEADONHATCH：孵化时死亡的胚胎数量
EYEDDEAD：达到眼发育期后死亡的胚胎数量
MALFNOEYEPROP：畸形胚胎占比
EYEDDEADPROP：达到眼发育期后死亡胚胎的占比
DEADONHATCHPROP：达到眼发育期后死亡胚胎的占比
FRYTOTPROP：成功孵化的卵子占初始卵子总数的比例
DDAY：以度日（degree days）表示的受精后时间