Whole-genome bisulfite sequencing

Raw reads from whole-genome bisulfite sequencing. Whole-genome bisulfite sequencing library preparations and Illumina sequencing of DNA samples from TET2 mutation carriers (Ly9, Ly11, Ly14, Id1) and their age-matched controls (Ly8, Ly10, Ly13, Id2, Id3) was done as a service at BGI (BGI Tech Solutions Co., Ltd., China). Bisulfite treatment was done with EZ DNA Methylation-Gold Kit (Zymo Research, CA, USA) for 300-400bp size-range fragments with methylated adapters in 5' and 3' ends. Sequencing was done with the HiSeq X-Ten platform using paired-end 150 base-pair read length.

原始全基因组亚硫酸氢盐测序数据。全基因组亚硫酸氢盐测序文库制备及对TET2突变携带者（Ly9、Ly11、Ly14、Id1）及其年龄匹配对照者（Ly8、Ly10、Ly13、Id2、Id3）的DNA样本进行Illumina测序，由北京基因组研究所（BGI Tech Solutions Co., Ltd., 中国）提供测序服务。使用EZ DNA Methylation-Gold试剂盒（Zymo Research，CA，美国）对300-400碱基长度的片段进行亚硫酸氢盐处理，5'和3'端带有甲基化适配器。测序采用HiSeq X-Ten平台，采用双端150碱基长度的读取长度进行测序。