Liver mRNA expression in high fat diet fed mice with hepatic specific Slug knockout

Liver steatosis is a driving force for nonalcoholic fatty liver disease (NAFLD), alcoholic liver disease, insulin resistance, and type 2 diabetes. Slug (also called Snai2 or Snail2) is a transcriptional regulator that may play a critical role in the regulation of liver function. To prove that, we used microarrays to detail the hepatic Slug regulated gene expression underlying liver steatosis and identified distinct pathways controlled by Slug during this process. C57BL/6J mice males with hepatic specific Slug knockout and their littermates were fed a high fat diet fed (HFD) for 11 weeks. Then RNA samples were extracted from the same parts of liver tissues and hybridizated on Affymetrix microarrays.