Use of genetics in monitoring control measures for Aedes aegypti and Aedes albopictus

This study evaluated the impact of long-term pyriproxyfen exposure on the genetic structure and diversity of Ae. aegypti and Ae. albopictus mosquito populations. The study was conducted in Manaus, Amazonas, Brazil, where pyriproxyfen dissemination stations have been monitored since 2014 up to the present day. Double digest restriction-site associated DNA sequencing (ddRADSeq) was performed. Sequencing quality control was applied with mean quality filtering, trimming, minimum length filtering and adapter removal procedures using Trimmomatic. All sequencing reads were aligned with BWA program to the reference genome of Ae. aegypti (NC_035159.1) and Ae. albopictus (JAFDOQ000000000.1). Variant calling was performed using Stacks v2.62, with gstacks approach. The variant panel was exported in the VCF v4.2 format. SNPs were removed from the variant panel based on a minimum allele frequency (MAF) < 0.1 and at least 8 non-missing genotypes within each group.本研究评估了长期暴露于吡丙醚（pyriproxyfen）对埃及伊蚊（Ae. aegypti）与白纹伊蚊（Ae. albopictus）种群遗传结构及多样性的影响。本研究于巴西亚马逊州马瑙斯市开展，该市自2014年起至今持续对吡丙醚投放站进行监测。本研究采用双酶切限制性位点相关DNA测序（double digest restriction-site associated DNA sequencing, ddRADSeq）技术，使用Trimmomatic软件完成测序质量控制，流程涵盖平均质量过滤、序列修剪、最小长度过滤及接头去除步骤。所有测序读段均通过BWA软件比对至埃及伊蚊参考基因组（NC_035159.1）与白纹伊蚊参考基因组（JAFDOQ000000000.1）。使用Stacks v2.62软件结合gstacks方法进行变异位点调用，变异位点集以VCF v4.2格式导出。基于最小等位基因频率（minor allele frequency, MAF）<0.1且每组内至少存在8个无缺失基因型的筛选标准，从变异位点集中移除单核苷酸多态性（single nucleotide polymorphism, SNPs）位点。