Data for: Lipidomics data showing different types of dietary fat and fructose interactions on the alteration of hepatic lipid composition in mice

These lipidomics data provide hepatic lipid profile induced by different types of dietary fat and fructose interactions, and are valuable for future studies on different fat sources derived high fat diet-induced nonalcoholic fatty liver disease (NAFLD) mouse models and underlying mechanisms. These lipidomics data showed hepatic fatty acid composition by individual lipid class, including free fatty acid (FFA), diacylglycerol (DAG), triacylglycerol (TAG), phospholipid (PL), and cholesteryl ester (CE). NAFLD is characterized with specific alteration of hepatic lipid composition. Fat and fructose are the two major components of western diet and often consumed together. Excess intake results in NAFLD. We hypothesize that different types of dietary fat in combination with fructose results in differential hepatic lipidomics profile which is associated with distinct metabolic phenotypes. Herein we present hepatic lipidomics data from adult male C57BL/6J mice. The mice were fed with high fat diet (42% of calories derived from fat) enriched with saturated fat (beef tallow), or omega-6 polyunsaturated fat (corn oil) or omega-3 polyunsaturated fat (menhaden fish oil) with or without fructose supplementation through 10% fructose (w/v) in the drinking water ad libitum for 20 weeks. Snap-frozen liver tissues were subjected to lipidomics analysis. Lipids were extracted from liver and individual lipid classes were separated by thin layer chromatography followed by methylation. The methylated fatty acids were extracted and analyzed by gas chromatography (GC).

本数据集提供了不同类型膳食脂肪与果糖相互作用诱导的肝脏脂质谱，可为后续针对不同脂肪源高脂膳食诱导的非酒精性脂肪性肝病（nonalcoholic fatty liver disease, NAFLD）小鼠模型及其潜在机制的研究提供宝贵参考数据。该脂质组学数据按单一脂质类别展示了肝脏脂肪酸组成，涵盖游离脂肪酸（free fatty acid, FFA）、二酰甘油（diacylglycerol, DAG）、三酰甘油（triacylglycerol, TAG）、磷脂（phospholipid, PL）及胆固醇酯（cholesteryl ester, CE）。非酒精性脂肪性肝病（NAFLD）以肝脏脂质组成的特异性改变为典型特征。脂肪与果糖是西方膳食的两大核心组成成分，且通常协同摄入，过量摄入可诱发NAFLD。本研究假设，不同类型的膳食脂肪与果糖联合作用时，会产生差异化的肝脏脂质组学谱，且该谱与独特的代谢表型密切相关。本数据集包含成年雄性C57BL/6J小鼠的肝脏脂质组学数据：实验小鼠被饲喂热量占比42%来自脂肪的高脂膳食，该膳食分别富含饱和脂肪（牛油，beef tallow）、ω-6多不饱和脂肪（玉米油，corn oil）或ω-3多不饱和脂肪（油鲱鱼油，menhaden fish oil），同时通过在饮用水中添加10%（w/v）果糖并以自由摄饮（ad libitum）的方式设置含果糖与不含果糖的干预组，喂养周期为20周。采集快速冷冻的肝组织进行脂质组学分析：先从肝脏中提取脂质，通过薄层色谱（thin layer chromatography）分离各单一脂质类别，随后进行甲基化修饰；提取甲基化脂肪酸后，采用气相色谱（gas chromatography, GC）进行分析。