ABUNDANCE OF HUMAN BETAHERPESVIRUSES IN SALIVARY GLAND LESIONS

Salivary gland tumours cause lesions with undefined aetiology. Human betaherpesviruses, such as Human cytomegalovirus (HCMV), human betaherpesvirus 6 (HHV-6), and Human betaherpesvirus 7 (HHV-7), may act as co-factors leading to the development of salivary gland neoplasms. The aim of this study was to investigate the presence of human betaherpesviruses (HCMV, HHV-6, and HHV-7) in salivary gland lesion samples.Samples from 68 patients with salivary gland lesions were tested (35 samples of mucocele, 27 of pleomorphic adenoma, and six of mucoepidermoid carcinoma). Following DNA extraction, multiplex quantitative polymerase chain reaction was used for quantification. Among the 68 samples, 51.4 % were mucocele; 39.7 %, pleomorphic adenomas; and 8.8 %, mucoepidermoid carcinomas. The abundance of betaherpesviruses in these lesions was high, presenting a detection rate of 52.9 %, 47.5%, and 39.7% for HCMV, HHV-6, and HHV-7, respectively. Overall, 60.0 % samples presented triple-infections with HCMV/HHV-6/HHV-7, 12.0 % were co-infections with HCMV/HHV-6 and HCMV/HHV-7, and 6.0 % were co-infections with HHV-6/HHV-7; maximum number of co-infections were detected in pleomorphic adenomas.HCMV, HHV-6, and HHV-7 are abundant in salivary gland lesions. High viral load can be useful for understanding their etiological role in such lesions.

涎腺肿瘤可引发病因未明的病变。人β疱疹病毒（Human betaherpesviruses）类群涵盖人巨细胞病毒（Human cytomegalovirus, HCMV）、人β疱疹病毒6型（Human betaherpesvirus 6, HHV-6）与人β疱疹病毒7型（Human betaherpesvirus 7, HHV-7），其可能作为辅助因子参与涎腺肿瘤的发生发展。本研究旨在探究涎腺病变样本中人β疱疹病毒（HCMV、HHV-6及HHV-7）的存在情况。本研究对68名涎腺病变患者的样本进行了检测，其中黏液囊肿（mucocele）样本35份、多形性腺瘤（pleomorphic adenoma）样本27份、黏液表皮样癌（mucoepidermoid carcinoma）样本6份。完成DNA提取后，采用多重定量聚合酶链反应（multiplex quantitative polymerase chain reaction）进行病毒载量定量。68份样本中，黏液囊肿占比51.4%、多形性腺瘤占比39.7%、黏液表皮样癌占比8.8%。上述病变中人β疱疹病毒的检出丰度较高，HCMV、HHV-6及HHV-7的检出率分别为52.9%、47.5%与39.7%。整体而言，60.0%的样本存在HCMV/HHV-6/HHV-7三重感染，12.0%为HCMV/HHV-6与HCMV/HHV-7双重感染，6.0%为HHV-6/HHV-7双重感染；其中多形性腺瘤样本的共感染检出数量最多。HCMV、HHV-6及HHV-7在涎腺病变中广泛存在，较高的病毒载量有助于阐明其在这类病变中的病因学作用。