AbsorbanceQ App for Generating Absorbance Images from Brightfield Image Captures

This page hosts the AbsorbanceQ app which generates absorbance images from brightfield image captures. Absorbance microscopy can be used to make trypan blue cell viability measurements more quantitative and traceable by measuring the moles of trypan blue in each dead cell. AbsorbanceQ was written in MATLAB and can be downloaded as a .zip file. When unzipped, the program will be installed and can run as a standalone .exe file with no dependencies beyond the operating system. This website includes images that can be downloaded to validate that AbsorbanceQ is functioning properly. References: [1] Babakhanova G, Zimmerman SM, Pierce LT, Sarkar S, Schaub NJ, Simon Jr CG (2021) Quantitative, Traceable Determination of Cell Viability Using Absorbance Microscopy, in press. [2] Babakhanova G, Zimmerman SM, Pierce LT, Sarkar S, Schaub NJ, Simon Jr CG (2021), Dataset for Absorbance Microscopy for Quantitative and Traceable Trypan Blue Cell Viability Measurement, National Institute of Standards and Technology, https://doi.org/10.18434/mds2-2347. [3] Zimmerman SM, Simon Jr CG, Babakhanova G (2021) AbsorbanceQ: An App for Generating Absorbance Images from Brightfield Images. J Res Natl Inst Stan 126:126039, https://doi.org/10.6028/jres.126.039.