Transcriptome analyses of iPSC-derived neural stem/progenitor cells and cerebral organoids from monozygotic twins discordant for schizoaffective bipolar disorder

To investigate the disease-associated differences in gene expression pattern during neuro developmental stage between the discordant twins, we performed RNA sequecing-based transcriptome analyses of iPSC-derived neural cells. For bulk RNA-seq, two clones of iPSC-derived neural stem/progenitor cells from each twin were analyzed. For single-cell RNA-seq, four clones of iPSC-derived cerebral organoids for each twin were analyzed. Two organoids for each clone were subjected to the analysis. [Ethics committee] Wako 1st Research Ethics Committee of RIKEN [Ethics approval number] Wako-1st-15-4(23)