Epigenetic Reactivation of CNS Endothelial Developmental Programs Triggers Adult Brain Angiogenesis, Promotes Post-Stroke Revascularization and Neuroregeneration.

We identified the role of brain endothelial cell Hdac2 and Ezh2 mediated epigenetic mechanism in maintaining blood brain barrier in adult mice. Also, we we studied the impact of reactivation of brain endothelial cell Wnt/ß-catenin signaling in adult mice.We evaluated derepression of Hdac2 mediated epigenetic mechanism in imice model of schemic stroke. Overall design: Ultra-low mRNA sequencing of FACS sorted CD31+ve/CD41-ve/CD45-ve adult mice brain cortical endothelial cells isolated from WT, Hdac2 ECKO, Ezh2 ECKO and ß-catenin GOF mice. For endothelial specific deletion of Hdac2 and Ezh2, or ß-catenin constitutive activation, Hdac2lox/lox:Cdh5CreERT2, Ezh2lox/lox:Cdh5CreERT2 and Ctnnb1lox/lox:Cdh5CreERT2 mice were treated with five doses of Tamoxifen (75mg/Kg body weight) and brains were harvested after 2 months.