Transcriptional Characterization of Early Esophageal Squamous Cell Carcinoma

To investigate the transcriptional characterization of early esophageal squamous cell carcinoma (ESCC), we performed RNA-sequencing of 10 matched pairs of tumor and adjacent normal tissues of early stage of ESCC patients Overall design: Total RNAs (500 ng) from ESCC tissues and matched normal tissues were extracted by Trizol® Reagent (Thermo, USA) and treated with DNase I (NEB) to remove DNA before constructing the RNA-seq libraries. Strand-specific RNA-seq libraries were prepared using the QIAseq FastSelect–rRNA HMR (Qiagen) and KAPA RNA HyperPrep (Roche) kits.The libraries were quality controlled with Qubit (Thermo Fisher Scientific, USA) and Qsep100 (BiOptic, China) and sequenced by the Illumina sequencing platform (Nova) on a 150 bp paired-end run.