Dataset for: Biological welding enables rapid and efficient bladder cystotomy closure and reveals the underlying repair mechanism

This dataset contains the processed gene expression count matrix (RNA-seq) generated for the study "Biological welding enables rapid and efficient bladder cystotomy closure".Due to archival hardware failure, the raw sequencing data (Fastq) generated in 2017 are no longer available. This repository provides the processed Read Count Matrix, which is sufficient to reproduce the differential expression analysis presented in the manuscript (Figures 7-10).Experimental Design:Organism:Canis lupus familiaris (Beagle dogs).Sample Prep: Total RNA was extracted from pooled bladder tissue samples (n=3 biological replicates pooled per library).Groups included in this dataset:1. Normal: Untreated/Normal bladder tissue (Baseline).2. Control: Traditional Suturing group (1 week, 4 weeks, 12 weeks post-operation).3. Welding: Biological Welding group (1 week, 4 weeks, 12 weeks post-operation).本数据集包含为研究《Biological welding enables rapid and efficient bladder cystotomy closure》（生物焊接可快速高效闭合膀胱切开术创口）所生成的经处理的RNA测序（RNA-seq）基因表达计数矩阵（gene expression count matrix）。因存档硬件故障，2017年生成的Fastq格式原始测序数据现已无法获取。本仓库提供经处理的读段计数矩阵（Read Count Matrix），可复现论文中展示的差异表达分析（differential expression analysis）结果（图7至图10）。 实验设计： 受试生物：家犬（Canis lupus familiaris，比格犬）。 样本制备：从合并的膀胱组织样本中提取总RNA，每份文库混合3份生物学重复样本。 本数据集包含以下分组： 1. 正常组：未处理的正常膀胱组织（基线对照）。 2. 对照组：传统缝合组（术后1周、4周、12周）。 3. 焊接组：生物焊接组（术后1周、4周、12周）。