Cross-platform Clinical Proteomics using the Charité Open Standard for Plasma Proteomics (OSPP)

we present the Charité Open Peptide Standard for plasma proteomics (OSPP), an open resource composed of 211 isotope-labeled peptides, intended to be used as an internal standard for plasma and serum proteomic projects. The OSPP was designed based on peptides consistently quantified across a panel of diverse human studies, is made of peptides that can easily be synthesized, that distribute equally over chromatographic gradients, and that show consistent identification performance across diverse LC-MS platforms, acquisition methods, and matrices. raw proteomics data have been deposited in the ProteomeXchange under accession code PXD070765

本研究提出面向血浆蛋白质组学的夏里特开放肽标准（Charité Open Peptide Standard for plasma proteomics, OSPP），该开放资源由211条同位素标记肽段组成，可作为血浆与血清蛋白质组学研究的内标使用。OSPP的设计依托于在多组多样化人类研究中可稳定定量的肽段，其包含的肽段均易于合成、在色谱梯度上分布均匀，且在多样化的液相色谱-质谱（LC-MS）平台、数据采集方法及样本基质中均能表现出稳定的鉴定性能。原始蛋白质组学数据已提交至ProteomeXchange数据库，登录号为PXD070765。