Expression of GITR correlates with MM clinical pathogenesis.

A) Expression of GITR was evaluated by Gene expression profiling analysis (GEO 2658 datestes). The average of GITR expression in MG, US and smoldering MM was labeled as red bar. B) Expression of GITR was evaluated by Gene expression profiling analysis (GEO 2658 datestes). The average of GITR expression in every MM subtype was labeled as red bar. >C) Patients belonging to Total Therapy 2 (TT2) and Total Therapy 3 (TT3) trials with available gene expression data were divided in poor and good prognosis groups based on the 70-gene index generated by the University of Arkansas for Medical Sciences (UAMS). The cutoffs for the poor prognosis groups were 0.66 and 0.79 for TT2 and TT3, respectively. Median normalized TNFRSF18 expression level was compared between good and poor prognosis groups. D) Kaplan-Meier curve showing survival of 414 MM patients in GITR high and GITR low groups. The two groups were divided by the median of GITR expression in GEP datasets (GSE2658 and GSE5900) and analyzed by graphpad software (P=0.03).