Supplementary dataset for Zhu et al. 2024: Tracing the evolutionary history of the temperature-sensing prion-like domain in EARLY FLOWERING 3 shows uniqueness of AtELF3

This record contains a supplementary dataset of sequence alignment files and a GWAS p-value table that support the publication: Zhu Z, Trenner J, Delker C, Quint M. 2024. Tracing the evolutionary history of the temperature-sensing prion-like domain in EARLY FLOWERING 3 shows uniqueness of AtELF3. (submitted to Molecular Biology and Evolution) Included files: 1. datasetFig1_ELF3EEC_tree_20240418_434seqs.fasta Multiple protein sequences alignment by MUSCLE (Edgar, 2004) of 434 ELF3/EEC protein sequences from 274 plant genomes, supporting the phylogenetic tree in Figure 1. 2. datasetFig2_ELF3EEC_alignment.fasta Multiple protein sequences alignment by MUSCLE (Edgar, 2004) of 69 ELF3/EEC protein sequences from selected species, supporting Figure 2. 3. datasetFig3_BrassicalesELF3_tree.fasta Multiple protein sequences alignment by MUSCLE (Edgar, 2004) of 52 ELF3 protein sequences from 44 Brassicales and 8 Poales species, supporting the phylogenetic tree in Figure 3. 4. datasetFig4_At319ELF3_CAAremoved_tree.fasta Multiple DNA sequences alignment by MUSCLE (Edgar, 2004) of 319 AtELF3 coding sequences (CAA repeats removed) supporting the phylogenetic tree in Figure 3. 5. pvalues_GWA_Portal_polyQ_length_vs_1001genomes_dataset_AMM.csv For a genome-wide association study (GWAS) the polyQ length of 319 Arabidopsis thaliana accessions was submitted as phenotype to the interactive GWA-Portal (Seren 2018, https://gwas.gmi.oeaw.ac.at). This P-value output file supports Supplemental Figure S10. Edgar RC (2004) MUSCLE: multiple sequence alignment with high accuracy and high throughput. Nucleic acids research 32: 1792-1797 Seren Ü. (2018). GWA-Portal: Genome-Wide Association Studies Made Easy. In: Ristova, D., Barbez, E. (eds) Root Development. Methods in Molecular Biology, Vol 1761. Humana Press, New York, NY.本数据集包含序列比对文件与全基因组关联分析（GWAS, Genome-Wide Association Study）p值表两类补充数据，用以支撑以下已投稿至《Molecular Biology and Evolution》的学术论文： Zhu Z, Trenner J, Delker C, Quint M. 2024. 追踪早花3基因（EARLY FLOWERING 3，ELF3）中温度敏感型朊病毒样结构域（prion-like domain）的进化历史，揭示拟南芥ELF3（AtELF3）的独特性。 （已投稿至《Molecular Biology and Evolution》） 包含文件如下： 1. datasetFig1_ELF3EEC_tree_20240418_434seqs.fasta 该文件为采用MUSCLE算法（Edgar, 2004）构建的多序列比对结果，涵盖来自274个植物基因组的434条ELF3/EEC蛋白序列，用于支撑图1的系统发育树。 2. datasetFig2_ELF3EEC_alignment.fasta 该文件为采用MUSCLE算法（Edgar, 2004）构建的多序列比对结果，涵盖来自筛选物种的69条ELF3/EEC蛋白序列，用于支撑图2。 3. datasetFig3_BrassicalesELF3_tree.fasta 该文件为采用MUSCLE算法（Edgar, 2004）构建的多序列比对结果，涵盖来自44个十字花目（Brassicales）与8个禾本目（Poales）物种的52条ELF3蛋白序列，用于支撑图3的系统发育树。 4. datasetFig4_At319ELF3_CAAremoved_tree.fasta 该文件为采用MUSCLE算法（Edgar, 2004）构建的DNA多序列比对结果，涵盖319条去除CAA重复序列的拟南芥ELF3编码序列，用于支撑图3的系统发育树。 5. pvalues_GWA_Portal_polyQ_length_vs_1001genomes_dataset_AMM.csv 本文件为全基因组关联分析的p值输出结果：将319份拟南芥（Arabidopsis thaliana）种质的多聚谷氨酰胺（polyQ）长度作为表型，提交至交互式分析工具GWA-Portal（Seren 2018, https://gwas.gmi.oeaw.ac.at）进行分析，该结果用于支撑补充图S10。 Edgar RC (2004) MUSCLE: multiple sequence alignment with high accuracy and high throughput. Nucleic acids research 32: 1792-1797 译：Edgar RC（2004）《MUSCLE：兼具高准确性与高通量的多序列比对工具》，《核酸研究》32卷：1792-1797 Seren Ü. (2018). GWA-Portal: Genome-Wide Association Studies Made Easy. In: Ristova, D., Barbez, E. (eds) Root Development. Methods in Molecular Biology, Vol 1761. Humana Press, New York, NY. 译：Seren Ü.（2018）《GWA-Portal：简化全基因组关联分析流程》，收录于Ristova D、Barbez E主编的《根系发育》，《分子生物学方法》丛书第1761卷，Humana出版社，美国纽约州纽约市。