Role of adipose tissue Na/K ATPase signaling in uremic cardiomyopathy

The objective of the present study is to explore the role of human MSC derived adipocytes transduced with/without lenti adipo-NaKtide or lenti adipo-scrambled NaKtide in experimental uremic cardiomyopathy. We investigated the pathophysiological changes in recipient mice that underwent partial nephrectomy surgery, as a model of uremic cardiomyopathy, and implanted with NaKtide transfected human MSC derived adipocytes. We aimed to demonstrate that adipocyte specific NaKtide reprograms the adipocyte phenotype and transplantation of these metabolically healthy adipocytes in diseased condition exhibits improved systemic and cardiovascular function, through intra-organ crosstalk. Overall design: RNA was extracted from Heart, Kidney and Visceral adipose tissues of SCID mice (12 weeks old, male) in each group; SCID-Sham, SCID-PNx, SCID-ControlCells-PNx, SCID-Lenti-NaKtideCells-PNx, SCID-Scrambled-NaKtideCells-PNx, and sequenced in a 2x50 base-pair design on an Illumina 1500 sequencer.