Raw Data from Fluoroacetate Dehalogenase Solved by Serial Oscillation Crystallography (1° oscillation per crystal), PDB 6MUH
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This is the raw data for PDB 6MUH: Fluoroacetate dehalogenase, room temperature structure solved by serial 1 degree oscillation crystallography. Data was taken at Beamline G3 of CHESS at 10.2 keV (1.2155 Å). Collection date was 2018-03-04. Beam size 7 µm V x 9 µm H, flux 2e11 ph/s. Detector: Dectris EIGER X 1M. Data format: Dectris HDF5 for EIGER. Rotation was counterclockwise about the laboratory Z-axis ( 0 -1 0 in the XDS coordinate system). Detector distance was 45 mm. One degree from each data well was collected, at 0.2° per frame. Each well has five data frames. Data was collected in four successive iterations, labeled 000-003, of 1600 wells; each *master.h5 corresponds to 8000 data frames.
本数据集为PDB(Protein Data Bank,蛋白质数据库)条目6MUH的原始数据,对应氟乙酸脱卤酶的室温晶体结构,通过连续1度振荡晶体衍射法解析获得。 实验数据采集于CHESS(Cornell High Energy Synchrotron Source,康奈尔高能同步辐射源)的G3光束线,X射线能量为10.2 keV(波长1.2155 Å),采集日期为2018年3月4日。光束尺寸为7 µm(垂直)×9 µm(水平),光子通量为2×10¹¹ 光子/秒。探测器为Dectris公司EIGER X 1M型探测器,数据格式为适配EIGER探测器的Dectris HDF5格式。扫描绕实验室Z轴(XDS坐标系下为(0, -1, 0))逆时针进行,探测器与样品的间距为45 mm。 每个样品采集孔的扫描角度范围为1度,每帧采集0.2度,每个孔包含5帧数据。本次数据采集分为4轮连续迭代,编号为000至003,每轮包含1600个样品采集孔;每个*master.h5文件对应8000帧数据。



