IMOS Biological Ocean Observer - sysdata.rda

This is the sysdata.rda file that is used to populate the IMOS Biological Ocean Observer (BOO) Shiny App. The IMOS biological and supporting data is accessed directly from the AODN, processed and reformatted to the requirements of the Shiny App and stored in this location.\nLineage: National Reference Stations\nWater samples are collected off small vessels at the IMOS National Reference Stations. The depth of the sample varies at each station. The sampling methods are fully described in the IMOS NRS Biogeochemical Operations Manual (Davies & Sommerville 2020). The analysis and quality control (QC) procedures (performed at CSIRO) are described in Eriksen et al. 2019. The SOTS data comes from the Southern Ocean Time Series project. The location is a fixed mooring with samples taken at various depths at regular intervals. Data sampling is being conducted as part of a larger IMOS monitoring program. The zooplankton samples are analysed at the Plankton Ecology Lab at CSIRO Marine and Atmospheric Research, Queensland. The samples are concentrated down to 100 ml in a measuring cylinder. A subsample (1 ml, 2.5 ml or 5 ml) is taken from the 100 ml concentrate using a stempel pipette. The sub sample is analysed to the lowest taxa possible. For each sample 100 adult copepods and 500 total zooplanktons must be counted. If these conditions are not met by the first sub sample analysis, a second sub sample is analysed. This continues until the two conditions are met. These counts are then converted to taxa / m3 and the analysis data is exported to IMOS.\n\nContinuous Plankton Recorder\nData sampling is being conducted as part of a larger IMOS monitoring program. The silk is removed from the CPR cassette and processed as described in Richardson et al 2006. The phytoplankton colour index (PCI) and the phytoplankton data are also analysed as per Richardson et al 2006. The zooplankton analysis is conducted differently to that described in Richardson et al 2006 as it is counted off the silk in a bogorov tray. This is accomplished by rinsing the silks in water and straining through a 10 micron mesh sieve. The collected plankton is transferred to a bogorov tray and counted under a disecting scope. This is done to retain the phytoplankton. AusCPR decided to analyse the zooplankton this way as it provides a more accurate analysis of the zooplankton present. It is easy to miss zooplankton when it is still on the silk and it is harder to identify. After counting the zooplankton and phytoplankton are transferred onto a preweighed filter and dried in an oven at 60 degrees C for 24-48 hours. Once dried the sample is reweighed to attain dry weight.

本数据集为sysdata.rda文件，用于填充IMOS生物海洋观测（Biological Ocean Observer, BOO）Shiny应用程序。IMOS生物及配套数据直接从澳大利亚海洋数据网络（Australian Ocean Data Network, AODN）获取，经处理与格式重构以适配该Shiny应用的需求后，存储于此路径下。
数据谱系：国家参考站（National Reference Stations）
水样采集工作在IMOS国家参考站的小型船舶上开展，各站位的采样水深存在差异。采样方法详见《IMOS NRS生物地球化学作业手册》（Davies与Sommerville, 2020）；由澳大利亚联邦科学与工业研究组织（Commonwealth Scientific and Industrial Research Organisation, CSIRO）完成的分析与质量控制（Quality Control, QC）流程则参见Eriksen等人2019年的研究成果。
南大洋时间序列（Southern Ocean Time Series, SOTS）项目的数据源自固定锚定站位，该站点按固定间隔采集不同深度的水样，采样工作作为IMOS更大规模监测计划的一部分实施。
浮游动物样品的分析工作在昆士兰州CSIRO海洋与大气研究中心的浮游动物生态学实验室开展。样品经浓缩至100ml量筒中，使用施泰因格莱普勒移液管（stempel pipette）从100ml浓缩液中提取子样品（体积为1ml、2.5ml或5ml）。对子样品进行分类鉴定至最低可区分类群，每份样品需计数100只成年桡足类与总计500只浮游动物。若首次子样品分析未满足上述两项计数要求，则需再次提取子样品进行分析，直至满足条件为止。最终将计数结果换算为类群数/立方米，并将分析数据导出至IMOS数据库。

连续浮游生物记录仪（Continuous Plankton Recorder, CPR）
数据采样工作作为IMOS更大规模监测计划的一部分实施。首先从CPR盒中取出滤绢，按Richardson等人2006年的方法进行处理；浮游植物颜色指数（Phytoplankton Colour Index, PCI）与浮游植物数据的分析流程同样遵循Richardson等人2006年的标准。但浮游动物的分析流程与Richardson等人2006年的描述存在差异：本次研究通过在博戈罗夫计数板（bogorov tray）上直接对滤绢上的浮游动物进行计数。具体操作如下：将滤绢置于水中冲洗，经10微米孔径筛网过滤收集浮游生物，随后将收集物转移至博戈罗夫计数板，在解剖镜下完成计数——该方法可保留浮游植物样本。AusCPR选择此种浮游动物分析方式，是因为其能更精准地鉴定采集到的浮游动物类群：若浮游动物仍附着在滤绢上，极易出现漏检且鉴定难度更高。完成浮游动物计数后，将浮游植物与浮游动物一同转移至预先称重的滤膜上，置于60℃烘箱中干燥24~48小时，待干燥完成后再次称重以获取样品干重。