Electrically evoked calcium waves and membrane oscillations reveal astrocytic excitability in the neurostimulation range

Electrically evoked calcium waves and membrane oscillations reveal astrocytic excitability in the neurostimulation range # Authors Miguel Aroso (1), Sara C Silva (1), Domingos L Castro (1), Paulo Aguiar (1,2,*)(1) Neuroengineering and Computational Neuroscience Lab (NCN), i3S - Instituto de Investigação e Inovação em Saúde, Universidade do Porto, Porto, Portugal(2) Faculdade de Medicina da Universidade do Porto, Porto, Portugal* Correspondence to: [e-mail] # Preprint https://doi.org/10.1101/2024.02.28.582466Currently (Apr 2026) under review in Journal of Neural Engineering # Overview This dataset contains recordings of calcium imaging and electrophysiology of rat primary astrocyte cultures, as described in https://doi.org/10.1101/2024.02.28.582466 # Folder Structure & Description The CalciumImaging folder contains representative TIFF/video files and associated metadata. All images were acquired at a sampling rate of 100 Hz. For all stimulated conditions, the electrical stimulus was applied 2 seconds after the start of the recording. This folder is organized into three subfolders: Evoked_Vs_Baseline: Comparative recordings of spontaneous vs. triggered activity- Basel.tif: Baseline recording (no stimulus).- Stim_800mV.tif: Recording with an 800 mV stimulusCa_Vs_NoCa: Experiments assessing the role of extracellular calcium.- Ca.tif: Stimulated recording (800 mV) in standard HBSS (with calcium).- No_Ca.tif: Stimulated recording (800 mV) in nominally calcium-free HBSS.400_800mV: Recordings testing stimulus-amplitude dependency.- The stimulation amplitude is indicated in the filename (e.g., 400mV.tif corresponds to a 400 mV stimulus). The Electrophysiology folder contains raw voltage recordings from astrocytes cultured on microelectrode arrays (MEAs). These recordings characterize the membrane voltage oscillations in response to electrical stimulation under controlled conditions and in the presence of pharmacological inhibitors. For all stimulated conditions, the initial stimulus was applied 1 minute after the start of the recording. This section contains subfolders labelled with abbreviated names of the chemical inhibitors used This folder contains the following subfolders: 400_800mV: Recordings testing stimulus-amplitude dependency.- TC_D1.h5: Recording with stimuli increasing amplitude. Stimuli were applied at 1-minute intervals, with each amplitude repeated three times. "D1" indicates the stimulation electrode. 800mV_3Stim: Recording assessing the reliability of astrocytic activation across repeated stimuli at 800 mV.- TS_A3.h5: Three 800 mV stimuli applied to electrode “A3”, interspaced by 1 minute, starting at the 1-minute mark. Ca_Vs_NoCa: Electrophysiological responses recorded in standard HBSS vs. nominally calcium-free HBSS.- Ca_A3.h5: Stimulated recording in standard HBSS- Ca_A3_NoCa.h5: Stimulated recording in nominally calcium-free HBSS. Cil (Cilnidipine): N-type and L-type VGCC blocker.- DC_A15.h5: control with response to stimulation at 800 mV.- DC_A15_Cil.h5: response to stimulation at 800 mV after Cilnidipine exposure. MA (Meclofenamic Acid): Inhibitor of gap junction communication.- DM_C16.h5: control with response to stimulation at 800 mV.- DM_C16_MA.h5: response to stimulation at 800 mV after Meclofenamic Acid exposure. Sur (Suramin): Purinergic receptor antagonist used to block ATP-mediated signaling.- DS_P16.h5: control with response to stimulation at 800 mV.- DS_P16_Sur.h5: response to stimulation at 800 mV after Suramin exposure. Ver (Verapamil): L-type VGCC blocker.- DV_O15.h5: control with response to stimulation at 800 mV.- DV_O15_Ver.h5: response to stimulation at 800 mV after Verapamil exposure.- Xest-C (Xestospongin C): Blocker of IP3 receptors.- DX_C16.h5: control with response to stimulation at 800 mV.- DX_C16_XC.h5: response to stimulation at 800 mV after Xestospongin C exposure.# 标题 电诱发钙波与膜电位振荡揭示神经刺激范围内星形胶质细胞的兴奋性 # 作者 米格尔·阿罗索（Miguel Aroso）(1)，萨拉·C·席尔瓦（Sara C Silva）(1)，多明戈斯·L·卡斯特罗（Domingos L Castro）(1)，保罗·阿吉亚尔（Paulo Aguiar）(1,2,*) (1) 神经工程与计算神经科学实验室（Neuroengineering and Computational Neuroscience Lab, NCN），葡萄牙波尔图大学健康研究与创新研究所（i3S - Instituto de Investigação e Inovação em Saúde, Universidade do Porto），波尔图，葡萄牙 (2) 波尔图大学医学院（Faculdade de Medicina da Universidade do Porto），波尔图，葡萄牙 * 通讯作者：[e-mail] # 预印本 https://doi.org/10.1101/2024.02.28.582466 （2026年4月当前正在《Journal of Neural Engineering》（神经工程期刊）审稿中） # 数据集概述 本数据集包含大鼠原代星形胶质细胞培养物的钙成像（calcium imaging）与电生理记录数据，相关细节参见https://doi.org/10.1101/2024.02.28.582466。 # 文件夹结构与说明 `CalciumImaging`（钙成像）文件夹包含代表性TIFF/视频文件及关联元数据。所有图像的采样率均为100 Hz。在所有刺激条件下，电刺激均于记录开始后2秒施加。 该文件夹分为3个子文件夹： 1. **Evoked_Vs_Baseline**：自发活动与触发活动的对比记录 - `Basel.tif`：基线记录（无刺激）。 - `Stim_800mV.tif`：施加800 mV刺激的记录文件。 2. **Ca_Vs_NoCa**：评估细胞外钙离子作用的实验 - `Ca.tif`：标准HBSS（含钙离子）中施加800 mV刺激的记录文件。 - `No_Ca.tif`：名义上无钙离子的HBSS中施加800 mV刺激的记录文件。 3. **400_800mV**：测试刺激幅度依赖性的记录 - 刺激幅度标注于文件名中（例如`400mV.tif`对应400 mV刺激）。 `Electrophysiology`（电生理）文件夹包含培养于微电极阵列（microelectrode arrays, MEAs）上的星形胶质细胞的原始电压记录数据。这些记录用于表征受控条件下及药物抑制剂存在时，星形胶质细胞膜电位对电刺激的响应振荡特性。在所有刺激条件下，初始刺激均于记录开始后1分钟施加。该部分包含以所用化学抑制剂缩写命名的子文件夹。 该文件夹包含以下子文件夹： 1. **400_800mV**：测试刺激幅度依赖性的记录 - `TC_D1.h5`：刺激幅度递增的记录文件。刺激以1分钟为间隔施加，每个幅度重复3次。“D1”代表刺激电极。 2. **800mV_3Stim**：评估800 mV下重复刺激时星形胶质细胞激活可靠性的记录 - `TS_A3.h5`：对电极“A3”施加3次800 mV刺激，间隔1分钟，首次刺激于记录开始后1分钟施加。 3. **Ca_Vs_NoCa**：标准HBSS与名义无钙离子HBSS中的电生理响应记录 - `Ca_A3.h5`：标准HBSS中的刺激记录文件。 - `Ca_A3_NoCa.h5`：名义无钙离子HBSS中的刺激记录文件。 4. **Cil（Cilnidipine，N型与L型电压门控钙通道阻滞剂）**： - `DC_A15.h5`：施加800 mV刺激的对照记录文件。 - `DC_A15_Cil.h5`：经西尼地平（Cilnidipine）处理后施加800 mV刺激的响应记录文件。 5. **MA（Meclofenamic Acid，间隙连接通讯抑制剂）**： - `DM_C16.h5`：施加800 mV刺激的对照记录文件。 - `DM_C16_MA.h5`：经甲氯芬那酸（Meclofenamic Acid）处理后施加800 mV刺激的响应记录文件。 6. **Sur（Suramin，ATP介导信号通路的嘌呤受体拮抗剂）**： - `DS_P16.h5`：施加800 mV刺激的对照记录文件。 - `DS_P16_Sur.h5`：经苏拉明（Suramin）处理后施加800 mV刺激的响应记录文件。 7. **Ver（Verapamil，L型电压门控钙通道阻滞剂）**： - `DV_O15.h5`：施加800 mV刺激的对照记录文件。 - `DV_O15_Ver.h5`：经维拉帕米（Verapamil）处理后施加800 mV刺激的响应记录文件。 8. **Xest-C（Xestospongin C，IP3受体阻滞剂）**： - `DX_C16.h5`：施加800 mV刺激的对照记录文件。 - `DX_C16_XC.h5`：经海绵抑素C（Xestospongin C）处理后施加800 mV刺激的响应记录文件。